Wire hanging test

Hanging test was used to assess strength and coordination. The mouse was placed on top of a wired mesh that was slowly turned over and maintained horizontally 35 cm above a thick layer of soft bedding. The length of time until the mice fell from the wire was recorded. Each testing consisted of three trials with one-minute intervals from which the timing scores were averaged. The ability to hang upside down and move around for 90 seconds was considered normal. Wild-type mice of different ages were able to hang and move around for at least 90 seconds. Untreated twi mice were unable to hang for any length of time after 25 days of age.