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MDA level was quantified by lipid peroxidation MDA assay kit (Abcam). In brief, cultured cells were harvested and washed using cold phosphate buffer solution (PBS; Solarbio). Then, cells were homogenized in Lysis Solution (Abcam), and insoluble material was removed by centrifuging. After that, thiobarbituric acid (TBA) was added into the wells and incubated at 95°C for 1 h. Supernatant was assessed by measuring the wavelength at 532 nm using a microplate reader (Thermo Fisher).
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