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Four micrometer-thick paraffin-embedded tissue sections were used for CD8 immunohistochemistry. Tissue sections were stained with monoclonal anti-CD8 antibody (clone C8/144B, 70306S) from Cell Signaling Technology. Lymphocytes with membranous staining were regarded as positive for CD8. All immunohistochemical sections were independently evaluated by two pathologists, and all evaluation scores were recorded. Two pathologists independently counted CD8+ cells and randomly selected 4–6 fields (200 ×) for each immunohistochemical section.
Copyright and License information: ©2021 Zhou, Huang, Xu, Lv, Wang, Zhan, Han, Shao, Lin, Lv and Song.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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