The cellular lipid contents of DIMFPs and DAFPs in 24-well plates were determined by Oil Red O staining. The staining steps were as follows: discard the cell culture medium, wash the adherent cells 3 times with PBS, fix the cells with 4% formalin for 30 min, wash the cells 3 times with PBS, and use Oil Red O (Wuhan AmyJet Scientific Inc., Wuhan, China) dyeing for 60 min. Subsequently, Oil Red O was discarded, and the cells were washed with PBS 3 times. After the water evaporated, isopropanol was added to extract Oil Red O for 10 min, and then, the absorbance of the solution was measured at 510 nm with a Multimode Microplate Reader (Thermo Fisher Scientific Inc., Suzhou, China).
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