2.5. Western blotting and quantitative real‐time (qRT)‐PCR analyses

Western blotting analyses were performed according to standard procedures using Immobilon‐P filters (Millipore), an enhanced chemiluminescence system (GE Healthcare), and antibodies listed in Table S2. qRT‐PCR analysis was also performed according to standard procedures, as described in Supplementary Methods, with the primers listed in Table S3. A TaqMan gene expression assay (Applied Biosystems) was also used to determine Ror1 (Mm00443463_m1), Ttf‐1(Mm00447558_m1), and 18s (Hs99999901_s1) mRNA expression.