Lymphocytes lysosomal membrane stability was determined from the redistribution of acridine orange as a fluorescent dye. Aliquots of the cell suspension (0.5 ml) that were previously stained with acridine orange (5 µM) were separated from the incubation medium by 1 min centrifugation at 1000 rpm. The cell pellet was then suspended in 2 ml of fresh incubation medium. This washing process was carried out twice to remove the fluorescent dye from the media. Acridine orange redistribution in the cell suspension was then measured fluorimetrically by the spectrophotometer set at 495 nm excitation and 530 nm emission wavelengths.
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