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Kato-Katz technique

MC Mamoudou Cisse
IS Ibrahim Sangare
AD Arthur D. Djibougou
MT Marc C. Tahita
SG Souleymane Gnissi
JB Jonathan K. W. Bassinga
SK Salifou Konda
AD Abdoulaye H. Diallo
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One Kato-Katz thick smear was prepared from each stool sample using a template holding 41.7 mg of stool sample [27]. The slides were left for 24 h to clear for easy visualization of S. mansoni eggs before being examined. Infection intensity of S. mansoni was estimated by multiplying the total number of eggs counted by 24, which was given as the eggs per gram (epg) of feces and classified as light, moderate and heavy per the threshold set by the World Health Organization [28]. Two independent laboratory technicians examined each slide, and any resulting discrepancies were resolved by the senior parasitologist. As a quality control check, 20% of all positive and negative slides were randomly chosen and re-read by the senior parasitologist to confirm positive or negative results.

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