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After diluting a small amount of stool (about 2 g) in 7 ml of 10% salt formalin solution (mixture of 100 ml pure formalin, 900 ml distilled water and 8.5 g sodium), the mixture was sieved in a centrifuge tube to remove large debris, and 3 ml of ether was added to it. The mixture was stirred vigorously for 30 s and centrifuged at 2000 rpm for 2 min. Then, the supernatant was discarded and the pellet was plated, using a pipette, between a slide and coverslip with lugol and read under a light microscope using 10× and 40× objectives [29].
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