Clonogenic Assay

The cells were seeded into 6-well plates at a density of 500 cells/well and cultured overnight. The cells were treated with different drug treatments for 24 h, and then the fresh medium was added into each well to replace the previous medium and incubated for one week. Colonies were fixed 4% paraformaldehyde and washed with phosphate-buffered saline (PBS) twice. The fixed cells were stained with 1% crystal violet for 10 min at room temperature and photographed. After imaging, a lysis buffer was used to dissolve the stains, and the absorbance at 630 nm was measured for quantitative analysis. Each experiment was done in triplicates.