Cell Lines

Fibroblasts were obtained from the dermis of healthy donors (normal fibroblasts, NF) and of a patient suffering from the dermatosparactic type of the Ehlers-Danlos syndrome (dermatosparactic fibroblasts, DF) (Nusgens et al., 1992). Cells were cultured in DMEM (Lonza) supplemented with 10% fetal bovine serum (FBS, Lonza). For collagen production, cells were cultured in DMEM supplemented with 1% FBS and 50 μg/ml 2-phospho-l-ascorbic acid (Sigma Cat#49752). The conditioned medium was collected after 48h, centrifuged for 15 min at 4,000 rpm at 4°C. The supernatant was conserved at −80°C before collagen purification.