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Quantitative Reverse Transcriptase PCR

ZZ Ziqi Zou
ML Mengyao Li
YZ Yunlian Zhou
JL Jiaying Li
TP Ting Pan
LL Lihua Lai
QW Qingqing Wang
LZ Lining Zhang
QW Qun Wang
YS Yinjing Song
YZ Yuanyuan Zhang
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Total RNA was extracted from cells using TRIzol reagent (Takara; Kyoto, Japan) according to the directions of the manufacturer. Single-strand cDNA was generated from total RNA using reverse transcriptase (Toyobo; Osaka, Japan). Real-time quantitative PCR analysis, using SYBR Green Master Rox (Roche), was performed as we previously described. The sequences for primers are shown in Supplementary Table 2. Data were normalized by the level of β-actin expression in each sample.

Copyright and License information:  ©2021 Zou, Li, Zhou, Li, Pan, Lai, Wang, Zhang, Wang, Song and Zhang.
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