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Hypoxia/Reoxygenation Model

JL Jun Li
CZ Cuiting Zhao
QZ Qing Zhu
YW Yonghuai Wang
GL Guangyuan Li
XL Xinxin Li
YL Yuhong Li
NW Nan Wu
CM Chunyan Ma
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The hypoxia/reoxygenation (HR) model was established as described previously (22). Specifically, when the cell density reached about 70%, cells were relocated to a tri-gas incubator with an atmosphere of 94% N2, 5% CO2, and 1% O2 and subsequently cultured with Earle's medium without glucose and FBS for 6 h to establish hypoxia. At the end of the incubation, the medium was replaced with DMEM supplemented with 10% FBS, and cells were transferred to CO2 incubator with an atmosphere of 5% CO2 for 3-h reoxygenation.

Copyright and License information:  ©2021 Li, Zhao, Zhu, Wang, Li, Li, Li, Wu and Ma.
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