Mouse cytokine array

The Proteome Profiler Mouse XL Cytokine Array Kit, (ARY028, R&D Systems) was used to quantify the 111 mouse proteins (cytokines, chemokines and growth factors) from gWAT of control (tamoxifen) and KO (≤ 10% body weight loss) mice according to the manufacturer's instructions as described previously⁷⁴. Proteome Profiler Mouse Cytokine Array Kit, Panel A (ARY006, R&D Systems) was used to quantify 33 chemokine and cytokines in plasma of control and KO ± Tofacitinib according to the manufacturer's instructions as described previously^{75, 76}. Briefly, gonadal adipose lysate/plasma was diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture was then incubated with the array membrane overnight at 4 °C. The membranes were washed and incubated with streptavidin–horseradish peroxidase followed by chemiluminescent detection. The array data were quantitated to generate a protein profile and results are presented as average signal (pixel density) of the pair of duplicate spots representing each cytokine or chemokine analyzed using Image-J software. The data presented is from three independent repeats.