2.16. RNA isolation and RNA sequencing

TRIzol reagent was used to extract total RNA. Then, the RNA concentration was detected using a spectrometer (Malcom: e‐spect). The amount of the precipitated mRNA was normalized to the input RNA fractions to eliminate possible differences in RNA sample preparation. RNA sequencing was performed by the Beijing Genomics Institute (BGI). Gene was considered significant when the Hochberg‐adjusted P value (P _adj.) was <.05 and the differentially expressed genes (DEGs) were subjected for enrichment analysis by the Gene Ontology (GO) and KEGG (Kyoto Encyclopedia of Genes and Genomes) tools. The RNA‐seq raw data were deposited to ArrayExpress under the accession number E‐MTAB‐9712.