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Transfection

ST Shiho Torii
CO Chikako Ono
RS Rigel Suzuki
YM Yuhei Morioka
IA Itsuki Anzai
YF Yuzy Fauzyah
YM Yusuke Maeda
WK Wataru Kamitani
TF Takasuke Fukuhara
YM Yoshiharu Matsuura
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The CPER products (25 μL out of a 50 μL reaction volume) without purification were transfected into HEK293-3P6C33 cells or BHK-21 cells with Trans IT LT-1 (Mirus), following the manufacturer’s protocols. At 6 hours post-transfection, the culture supernatants of HEK293-3P6C33 cells were replaced with DMEM containing 2% FBS and doxycycline hydrochloride (1 μg/ml), and BHK-21 cells were overlaid by VeroE6/TMPRSS2 cells.

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