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HLC was performed by a pair of trained collectors from 1800 until 0000 h each night. The same pair of collectors performed the HLC each night to prevent collection bias. All mosquitoes that landed on the bare legs were caught using 50 × 19 mm glass vials (Fig. 2d). The glass vials contained a small damp tissue at the base to keep the environment inside the vial humid. Once the mosquito was trapped, the vial was plugged with cotton wool. The mosquitoes were separated based on the hours of collection.
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