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Alizarin Red and Calcein Double Labeling

XH Xiangru Huang
AJ Anting Jin
XW Xijun Wang
XG Xin Gao
HX Hongyuan Xu
MC Miri Chung
QD Qinggang Dai
YY Yiling Yang
LJ Lingyong Jiang
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Mice received injections of calcein (Sigma, Cat#: C0875) and Alizarin Red (Sigma, Cat#: A5533) 7 and 3 days before euthanasia, respectively. Isolated femurs and tibias were dehydrated and embedded in polymethylmethacrylate. Specimens then were cut into continuous 5-μm thick sections with a rotary microtome. Images were captured as previously described (Yang et al., 2020).

Copyright and License information:  ©2021 Huang, Jin, Wang, Gao, Xu, Chung, Dai, Yang and Jiang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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