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Microscopy and TEM observation

FY Fang Yan
ZG Zhehao Gong
GH Guojian Hu
XM Xuesong Ma
RB Runyao Bai
RY Ruonan Yu
QZ Qiang Zhang
WD Wei Deng
ZL Zhengguo Li
HW Hada Wuriyanghan
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For histological analysis, tissue samples were collected from the pedicel at 25 dpa and immersed in FAA solution, placed under vacuum for 15 min, and incubated at 25 °C for 72 h. The samples were dehydrated, stained, and observed under a microscope according to the literature32. At anthesis, flowers of WT plants and SlBL4 RNAi line tomatoes were emasculated and subsequently counted and sampled for anatomical assessment at 10 dpa. For the histological assessment of SlBL4pro::GUS expression, tissue samples were collected from the pedicel of pSlBL4-GUS transgenic tomato at the following times: at 6, 4, 2 dba; on the anthesis day; and at 2, 25 dpa. The pedicel of the AZ at 25 dpa was observed by transmission electron microscopy (TEM) according to the literature34.

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