Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Oxidase-like and peroxidase-like property of PtCo@G in PBS (pH 1.0)

LZ Lufeng Zhang
LZ Liang Zhang
HD Hui Deng
HL Huan Li
WT Wentao Tang
LG Luyao Guan
YQ Ye Qiu
MD Michael J. Donovan
ZC Zhuo Chen
WT Weihong Tan
request Request a Protocol
ask Ask a question
Favorite

For assay of the product O2•−, hydroethidine (HE) was used as a probe, which could be easily oxidized by O2•− to generate the highly fluorescent product ethidium (E+). In a typical procedure, HE was added into the reaction solution (PBS, pH 1.0) for 30 min, and then the fluorescence spectra of the samples were collected using fluorescence spectroscopy (HORIBA Fluoromax-4, USA).

For assay of the product •OH, TA, and DEPMPO, which were used as probes, could easily react with •OH to form a highly fluorescent product (TAOH) and EPR spin adduct (DEPMPO-OH). In experimental procedure, TA and DEPMPO were added into reaction solutions (PBS, pH 1.0). Then, the fluorescence spectra and EPR of the samples were performed by fluorescence spectroscopy (HORIBA Fluoromax-4, USA) and EPR spectroscopy (JEOL, Japan).

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A