Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

DPPH radical scavenging assay

BD Bhanuz Dechayont
PP Pathompong Phuaklee
JC Jitpisute Chunthorng-Orn
TJ Thana Juckmeta
OP Onmanee Prajuabjinda
KJ Kitrawee Jiraratsatit
ask Ask a question
Favorite

A stock solution of DPPH solution in absolute EtOH (6 × 10− 5 M) was freshly prepared and protected from light [17]. The herb extracts were prepared in a serial dilution (5 concentrations). The extracts 100 μL were individually added and mixed with an equal volume of DPPH solution in 96-well microplates and maintained in the dark at room temperature for 30 min. The absorbance was measured at 520 nm. The scavenging activity was calculated as a percentage inhibition in the formula (3) below:

The IC50 was calculated from a dose-response curve using GraphPad 4.03 software.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A