Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

ABTS radical cation decolorization assay

BD Bhanuz Dechayont
PP Pathompong Phuaklee
JC Jitpisute Chunthorng-Orn
TJ Thana Juckmeta
OP Onmanee Prajuabjinda
KJ Kitrawee Jiraratsatit
request Request a Protocol
ask Ask a question
Favorite

The ABTS reagent was prepared by mixing ABTS 7 mM concentration with 2.45 mM potassium persulfate in deionized water and left in the dark at room temperature for 12–16 h [21]. The mixture reagent was diluted with deionized water to give an absorbance of 0.700 ± 0.020 at 734 nm. Serial dilutions of the herbal extracts were made in absolute EtOH for the ethanol extracts and distilled water for the aqueous extracts. ABTS reagent (180 μL) was mixed with 20 μL of sample solution at five concentrations and the absorbance was measured after exactly 6 min at 734 nm in 96-well microplates to determine the scavenging activity. All determinations were carried out in triplicate. The scavenging activity was calculated as a percentage inhibition in the formula (4) below.

The IC50 was calculated from a dose-response curve using GraphPad 4.03 software.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A