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PRF Protocol

SB Sebastian Blatt
DT Daniel G. E. Thiem
SK Solomiya Kyyak
AP Andreas Pabst
BA Bilal Al-Nawas
PK Peer W. Kämmerer
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For the PRF protocol, blood was collected from three healthy volunteers who gave their informed consent to this study in accordance with the ethical standards of the National Research Committee (Ärztekammer Rheinland-Pfalz, no. “2019-14705_1”) and the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Ten milliliters of peripheral venous blood per sample were collected after puncturing the cephalic or the median cubital vein with the vacutainer system and specific sterile plain vacuum tubes with additional silicone within their coating surface for solid (A-PRF+, Mectron, Carasco, Italy) and liquid PRF, respectively (iPRF, Mectron, Carasco, Italy). Next, PRF was directly manufactured (1,200 rpm for 8 min, relative centrifugal force 177 g at a fixed angle rotor with a radius of 110 mm, Duo centrifuge, Mectron, Carasco, Italy), as previously described (Blatt et al., 2020).

Copyright and License information:  ©2021 Blatt, Thiem, Kyyak, Pabst, Al-Nawas and Kämmerer.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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