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ELISA for CTx, osteocalcin, RANKL, and OPG

WL Wei Li
CW Chuan Wei
LX Lei Xu
BY Beibei Yu
YC Ying Chen
DL Di Lu
LZ Lina Zhang
XS Xian Song
LD Liyang Dong
SZ Sha Zhou
ZX Zhipeng Xu
JZ Jifeng Zhu
XC Xiaojun Chen
CS Chuan Su
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To quantify the levels of CTx, osteocalcin, RANKL, and OPG in serum or BMp, RATlaps ELISA kit (catalog number AC-06F1, Immunodiagnostic Systems, Fountain Hills, AZ), mouse osteocalcin ELISA kit (catalog number 60–1305, Immutopics, San Clemente, CA), mouse TRANCE/TNFSF11/RANK Ligand ELISA kit (catalog number DY462, R&D Systems, Minneapolis, MN), and mouse osteoprotegerin/TNFRSF11B immunoassay ELISA kit (catalog number MOP00, R&D Systems) with established protocols from the manufacturer were used, respectively. CTx, osteocalcin, RANKL, and OPG were measured in serum or BMp from mice, following an overnight fast.

Copyright and License information:  ©2021 Li et al ©2021 Pearce, HerbertThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Pearce, HerbertThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Pearce, HerbertThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Pearce, HerbertThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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