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The levels of extracellular DNA in supernatants were quantified using Quant-iT PicoGreen dsDNA assay kit according to the manufacturer’s instructions. PicoGreen is a cell-impermeable dye that binds to extracellular dsDNA without staining live cells. Fluorescence intensity was measured on a SpectraMax M3 (Molecular Devices) fluorescent plate reader at an excitation wavelength of 480 nm and an emission wavelength of 520 nm, with a 515-nm emission cutoff filter. The calibration curve was constructed using a standard dsDNA of a known concentration.
Copyright and License information: ©2017 Wan, Zhao, Fan, Hu and Jin.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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