Glucose consumption assay

To measure the glucose uptake was performed using the glucose uptake kit (Cayman Chemicals, Ann Arbor, MI) as per manufacturer's protocol. Briefly, cells were seeded at a density of 5000 cells/well in 96-well plates. Under various experimental conditions, cells were cultured along with 2-NBDG, a fluorescent analog of 2-deoxy glucose. The quantity of glucose consumption of the tested cancer cells was detected by fluorimetric analysis (485/535 nm) according to the manufacturer's instructions using Spectra Max Plus (Molecular Devices, Sunnyvale, CA).