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HIV gag p24 protein in samples from either precleaned or IP eluant was measured according to our previously published methods (Wu et al., 2017) on the Quanterix HD-X platform. p24 Simoa kit was obtained from Quanterix (Billerica, MA). p24 concentrations were calculated from raw signal average enzyme per bead (AEB) by the instrument’s software with four-parameter logistic regression curve fitting, and p24 levels were reported as pg/million cells or pg/mL in cell lysate and CM (Wu et al., 2017, 2021).
Copyright and License information: ©2021 Wu, Cheney, Huang, Hazuda, Howell and Zuck.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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