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Statistical Analysis

KH Kaoru Hatate
JR J. Hunter Rice
KP Karsten Parker
JW J. Jayne Wu
AT Amy Turner
JS Judith R. Stabel
SE Shigetoshi Eda
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Sample-to-positive (S/P) ratios were calculated according to the following equation:

where “value” refers to either RR or OD. Pearson's correlation analysis and Mann–Whitney U test were performed in Excel and R, respectively. The LOD was estimated as described previously (27, 28). Briefly, the RR or OD values were plotted against the HRP concentrations (1–4 pM and 1–16 pM, respectively). A linear regression generated in Excel was used to find the values of slope and y-intercept of the line of best fit. The SD of the data was multiplied by 3 and added to the y-intercept, which was then used to find the LOD (i.e., the corresponding concentration of HRP on the linear regression line).

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