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Extracellular Acidification Rate (ECAR) Assay
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Seahorse XF-96p analyzer was used to detect real-time status changed of ECAR. GC cells with miR-365a-3p mimics or NC were seeded into XF96 cell culture microplates at 40000 cells/well and cultured for 24 h. Then replaced medium with Seahorse DMEM containing 2 µM glutamine, and the microplates were maintained in a non-CO2 incubator at 37°C for 60 min. The data were analyzed to cell numbers and plotted as ECAR (mpH/min) as a function of time.
Copyright and License information: ©2021 Yang, Liu, Han, Qiu, Wang and Wang
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