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4.9. Hematoxylin-Eosin Staining

HH Hideyuki Hara
JC Junji Chida
AP Agriani Dini Pasiana
KU Keiji Uchiyama
YK Yutaka Kikuchi
TN Tomoko Naito
YT Yuichi Takahashi
JY Junji Yamamura
HK Hisashi Kuromatsu
SS Suehiro Sakaguchi
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Paraffin-embedded samples were sectioned at 5 µm. The sectioned samples were deparaffinized, rehydrated, and stained with Mayer’s hematoxylin solution (131-09665; Wako Pure Chemical Industries) at RT for 5 min and 1% eosin Y solution (051-06515; Wako Pure Chemical Industries) at RT for 2 min. After washing, the samples were mounted with Softmount (192-16301; Wako Pure Chemical Industries). Images of sample were visualized using BZ-810 (Keyence, Osaka, Japan) and analyzed with BZ-800 analyzer software (Keyence).

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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