TEM was used to assess mitophagy. Briefly, BV2 cells were cultured and treated as described above. After OGD treatment, BV2 cells were fixed with 2.5% glutaraldehyde for 2 h at 37 °C and 1% osmium tetraoxide for 1 h. Thereafter, BV2 cells were subjected to gradient dehydration in ethanol and embedded in Spurr resin. Next, cells were cut into 70-nm ultrathin sections and collected on nickel grids, followed by staining with uranyl acetate and lead citrate. Finally, cells were photographed using a transmission electron microscope (JEM-1230, JEOL, Japan), and the images were analyzed with ImageJ software.
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