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2.7. Dihydroethidium (DHE) Staining for ROS Production

SO Su-Yeon Oh
YK Yun-Ho Kim
MK Min-Kyung Kang
EL Eun-Jung Lee
DK Dong-Yeon Kim
HO Hyeongjoo Oh
SK Soo-Il Kim
WN Woojin Na
IK Il-Jun Kang
YK Young-Hee Kang
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Paraffin-embedded tissue sections of airways were prepared for DHE staining [30]. Airway tissues were stained by incubating for 1 h in 20 μM DHE (Invitrogen, Carlsbad, CA, USA). For the identification of nuclei, 4′,6-diamidino-2-phenylindole (DAPI) was given for 10 min. Stained tissues on slides were mounted and images were taken using an optical microscope (Carl Zeiss).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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