A volcano plot was used to examine the differences/fold changes of the genes from the mean values. Those with significant changes in transcriptome profiles were analyzed using the CLC Genomics Workbench (CLC Bio, Aarhus, Denmark). Differences in genes were shown in a 2D scatter plot. Gene expressions were based on log2 ratio in the x-axis, and the p-value was represented as −log10. The genes colored with blue were significantly downregulated (p ≤ 0.05; z-score ≤ −2) and the genes colored with red were significantly upregulated (p ≤ 0.05; z-score ≥ 2).
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