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Cell adhesion and proliferation assessment

LZ Lan Zhang
JZ Juan Zhang
FD Fang Dai
YH Yong Han
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L-929 cells with a density of 2 × 104 cells per cm2 were seeded on each sample and incubated for 1 h, and 1, 3, and 7 days. After each incubation period, the adhesion and proliferation of L-929 were evaluated by MTT assay, and absorbance was measured by a Multiscan Spectrum (Multiskan FC; Thermo, America). Four specimens for each group were tested.

After incubation for 1 and 3 days, cells were washed with phosphate buffered saline (PBS), fixed with 2.5% glutaraldehyde, dehydrated in graded ethanol, dried in vacuum overnight, and finally coated with gold to be observed by FESEM. The viability of cells were also evaluated by a live/dead viability/cytotoxicity kit (Invitrogen, Eugene, OR) and finally observed by an Olympus BX52 microscope.

Copyright and License information: The Author(s) ©2017
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