2.4. LMNA Variant Analysis

DNA was prepared from peripheral white blood cells with standard procedures [24]. LMNA exons 1–12 and the surrounding intronic sequences were amplified by PCR using primers and conditions available upon request. After purification with ExoSAP-IT^TM (Applied Biosystems, Waltham, MA, USA), the PCR products were directly sequenced with the same primers used for amplification and the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems). Electropherograms were produced with an ABI 3100 automated sequencing analyzer (Applied Biosystems), and analyzed using DNA Baser (2020, Heracle BioSoft v.5.8.0, www.dnabaser.com, accessed on 17 March 2021).