Recombinant TTR variants, recombinant Apolipoprotein E (ApoE) ε3 (1-299), and synthetic human IAPP 1-37 were obtained from AlexoTech AB (Umeå, Sweden). The quality of the proteins was verified by LC-MS before analysis. The employed IAPP contained a disulfide bridge between the positions Cys2 and Cys7 and was amidated at its C-terminal.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.