Determination of cholesterol in mouse plasma

ND Noton K. Dutta
NB Natalie Bruiners
MP Michael L. Pinn
MZ Matthew D. Zimmerman
BP Brendan Prideaux
VD Véronique Dartois
MG Maria L. Gennaro
PK Petros C. Karakousis
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Plasma was collected in heparin-containing tubes directly from the heart at the time of sacrifice. Total-cholesterol and LDL-cholesterol were measured using the Roche Cobas® Cholesterol Gen.2 (catalogue number 05168538190) and the LDL-Cholesterol plus 2nd generation (catalogue number 05171369190) assays and a Roche Cobas® c701 analyser. Both analytes were measured photometrically following enzymatic conversion of substrates.

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