Plasma was collected in heparin-containing tubes directly from the heart at the time of sacrifice. Total-cholesterol and LDL-cholesterol were measured using the Roche Cobas® Cholesterol Gen.2 (catalogue number 05168538190) and the LDL-Cholesterol plus 2nd generation (catalogue number 05171369190) assays and a Roche Cobas® c701 analyser. Both analytes were measured photometrically following enzymatic conversion of substrates.
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