2.4.2. Measurement of Particle Size and Zeta Potential of Liposomes

The particle size and polydispersity index (PDI) of liposomes were determined by dynamic light scattering (DLS) using a Zetasizer Nano ZSP (Malvern Instruments, Worcestershire, UK). Liposome powder was rehydrated with deionized water to its original volume and further diluted tenfold with a 10% sucrose solution. Samples were analyzed at a detection angle of 173° and a recording temperature of 25 °C. The zeta potential of liposomes was measured by laser doppler microelectrophoresis on the Zetasizer Nano ZSP. Malvern Zetasizer DTS software (version 7.12) was used for data acquisition and analysis. All measurements were carried out in triplicate.