2.4. Circular Dichroism Spectroscopy

The circular dichroism (CD) spectra were acquired on the Jasco J-715 CD spectrometer (Easton, MD USA) collecting 64 scans (4 s averaging time) for each spectrum and using a quartz cuvette with a 1 cm path length. The measurements were performed on samples containing peptides (5 µM) in buffer (5 mM phosphate, 10 mM NaCl, pH 7.0) in the presence and in the absence of vesicles (250 µM of 100% POPC). Care was taken that the sample absorbance never exceeded 1.5 at all wavelengths to produce reliable ellipticity values. Mean residue ellipticity (MRE; deg × cm² × dmol⁻¹) values were calculated using the following equation, where θ is ellipticity (mdeg), l is pathlength (cm), C is peptide concentration (M), and N is number of residues.