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2.3. Encapsulation Efficiency and Drug Loading Capacity

ZC Zhuo Chen
ZF Zhinan Fu
LL Li Li
EM Enguang Ma
XG Xuhong Guo
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The encapsulation efficiency (EE) and drug loading capacity (DLC) of the CUR-loaded NPs were determined using an ultraviolet-visible spectrophotometer. A certain amount of nanosuspension after dialysis was dissolved in a mixed solution of dichloromethane and methanol with the volume ratio of dichloromethane to methanol of 3:1. The concentration of CUR in organic solvent was measured by ultraviolet-visible spectrophotometer UV-2550 (Shimadzu Inc., Shanghai, China), and the characteristic peak of absorption in CUR located at 423 nm. The encapsulated CUR concentration was determined from the linear regression of the absorbance versus the concentration of CUR. The concentration of CUR in NPs can be calculated by comparing the CUR standard curve in dichloromethane methanol solution. The EE and DLC were calculated by the equations as follows:

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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