3.2.5. Neurotoxicity Screening (NT)—Rotarod Test

In this test, mice were trained to balance on an accelerating rod that rotated at 10 rotations per minute (Rotarod apparatus, May Commat RR0711, Turkey; rod diameter: 3 cm). During the training session, the animals were placed on a rotating rod for 3 min with an unlimited number of trials. Proper experiment was conducted at least 24 h after the training trial. On the test day, trained mice were intraperitoneally pretreated with the test compound and were evaluated in the rotarod test (at the screening dose of 100 mg/kg just before the MES test or at a dose of 300 mg/kg after 0.5 h). Neurotoxicity was indicated by the inability of the animal to maintain equilibration on the rod for 1 min.