2.4. Expression Vector Constructs and Stable Transfection

The expression vector containing the coding region of human MALT1 cDNA (HG11618-UT) and control pCMV3 vector were purchased from Sino Biological Inc. (Beijing, China). The pCMV-IκBαM, an inhibitor of NF-κB expression vector, contains two mutations at residues 32 and 36 of serine to alanine that prevent phosphorylation (Cat. no. 631923; Clontech Laboratories, Inc.; Mountain View, CA, USA). The constructs of NIK (MAP3K14) expression vector (pcDNA-NIK) and control pcDNA3 expression vector were described in the previous studies [31]. The expression vectors were introduced into prostate carcinoma cells by electroporation using the ECM 830 (BTX, Holliston, MA, USA) set on a single pulse setting, 70 msec, and 180 V. The mock-transfection cells were transfected with control pCMV3 or pcDNA3 expression vectors and were clonally selected in the same manner as the gene-overexpression cells.