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2.2. Total Genomic DNA Extraction
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Total genomic DNA (gDNA) was extracted from the skeletal muscle using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions [18]. The extracted gDNA was quantified using Nanodrop (Thermo Fisher Scientific, Waltham, MA, USA). The extracted DNA had a concentration of 40 ng/µL, while the purity range was in the ratio of 1.8–2.0 at A260/A280. Electrophoretic analysis in 1% agarose gel was performed to verify DNA integrity.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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