2.10.3. ABTS Assay

ABTS assay was based on a previously published method [23] with some modifications [24], using Trolox^®, a water-soluble analogue of vitamin E, as standard. ABTS^•+ radical cation was produced by soluble of 10 mg solid ABTS in mixture of water and 2.4 mM potassium persulfate aqueous solution (final concentration of ABTS 2 mM). This stock solution was kept in the dark at room temperature for 12–16 h for incubation. The ABTS^•+ solution was then diluted with distilled water to obtain an absorbance of 1.05 ± 0.05 at 734 nm. After 240 μL sample, solvent or Trolox standard solution was added to 2.88 mL ABTS^•+ solution; absorbance was measured at 10 min. Results of the ability of dendrimers to scavenge of cation radical ABTS are presented as Trolox equivalent antioxidant capacity (TEAC) and as IC₅₀ parameter. All analyses were performed in triplicate and the results were expressed as the mean value ± standard deviation.