Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.4.7. Expression of Occludin and Zona Occludens 1(ZO-1) in Intestine

XL Xin-Ran Liu
NZ Na Zhu
YH Yun-Tao Hao
XY Xiao-Chen Yu
ZL Zhen Li
RM Rui-Xue Mao
RL Rui Liu
JK Jia-Wei Kang
JH Jia-Ni Hu
YL Yong Li
request Request a Protocol
ask Ask a question
Favorite

Expression of occludin and ZO-1 in the intestine were detected by immunohistochemistry, using rabbit polyclonal antibody against occluding (BIOSS, Beijing, China, 1:200 dilution) and ZO-1 (BIOSS, China,1:500 dilution). Slides were evaluated by Image-Pro Plus version 6.0 (Media Cybernetics Inc., Bethesda, MD, USA), calculating the mean of optical density.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A