2.5. RAPD PCR

Randomly amplified polymorphic DNA polymerase chain reaction (RAPD PCR) was performed using the phage lysate as template. The phage lysate was prepared with 85 µL phage stock (10¹⁰–10¹¹ pfu/mL), treated with 10 µL 10XDNase buffer (100 mM Tris-HCl, 25 mM MgCl₂, pH 7.5) and 5 µL 10 mg/mL DNase I (SIGMA DN25), incubated at 37 °C shaking (300 rpm) for 60 min, followed by a second incubation at 75 °C for 10 min. Four oligonucleotides were designed [38] for PCR using MyTaq Red Mix 1X (Bioline, Cat#BIO-25043), according to the manufacturer’s recommendations. The oligonucleotides used were OPL5, RAPD5, P1 and P2 (Table S1), as previously described [38]. The PCR products were analysed by 0.8% agarose-gel electrophoresis with 1 kb ladder (Bioline, Cat#H1-819101A), run at 100 V for 60 min, and visualised with Midori Green DNA/RNA staining (Nippon Genetics, Cat#MG06).