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Lactate dehydrogenase (LDH) cytotoxicity assay

CL Chun Hwee Lim
MP Manoj Puthia
MB Marta Butrym
HT Hui Min Tay
ML Michelle Zi Yi Lee
HH Han Wei Hou
AS Artur Schmidtchen
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Cytotoxic effects of TCPs were assessed with the LDH release assay. PMNs were first treated with the indicated concentrations of IVE25 or GKY25 for 1 hr at 37 °C on a 96-well flat-bottom plate. The supernatants were then used for LDH assay according to manufacturer’s instructions. Absorbance readings at 490 and 680 nm were acquired using the Cytation 3 Cell-Imaging Multi-Mode reader (Research Instruments, USA). LDH activity was determined by subtracting absorbance reading at 680 nm from the reading at 490 nm (A490 − A680). Per cent cytotoxicity was calculated as below:

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