Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Next-generation sequencing.

TB Thijs Bosch
SW Sandra Witteveen
AH Anja Haenen
FL Fabian Landman
LS Leo M. Schouls
request Request a Protocol
ask Ask a question
Favorite

All 206 LA-MRSA isolates included in this study were subjected to NGS. In total, 148 LA-MRSA isolates, originating from the 3 predominant LA-MRSA MLVA/spa types, the previously described outbreak (23), the presumed LA-MRSA outbreak, and the 5 presumed nosocomial transmission events (16), were sequenced as part of the 100K genome project by University of California—Davis on a HiSeq 2000 (http://100kgenome.vetmed.ucdavis.edu/). The other 58 isolates were commercially sequenced on a HiSeq 2500 sequencer (BaseClear, Leiden, Netherlands).

Copyright and License information:  ©2016, American Society for Microbiology. All Rights Reserved.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A