Maize plants were dissected 45 days after infestation, which would ensure all larvae of the multivoltine individuals had completed development to pupation. The number of larvae and pupae were recorded separately. Diapausing larvae were counted as univoltine individuals whereas pupae were counted as multivoltine individuals.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.