2.5. Ammonium Sulfate Precipitation

Western blot analysis indicated that following centrifugation at 13,000× g for 10 min, the VLP 3 was found in the soluble fraction (supernatant) and VLP 1 was found in the insoluble fraction (pellet). The VLP 1 pellet was resuspended in renaturing buffer, 0.1 M tris buffer pH 8.7, 1 mM EDTA, 0.15 M NaCl. The soluble fraction from the VLP 3 reaction and the resuspended pellet of the VLP 1 reaction were each combined in a 1:1 ration with 1.9 M ammonium sulfate and allowed to incubate for 5 min. After the particles were pelleted by centrifugation at 13,000× g for 10 min, they were resuspended in renaturing buffer.