2.6. Behavioral Parameters

On days 1st, 13th, 23rd, 43rd, the locomotion was monitored by actophotometer (INCO (instruments and chemicals private limited), Haryana). Motor activity detected in the device through photocells. Before the recording, the animals were individually placed in the activity room for three minutes forhabituation. Over five minutes, each animal was seen and shown as a count for five minutes [60].

The Morris Water Maze (MWM) was used to test escapelatency on days 40th, 41st, 42nd, 43rd, and the time spent in the target quadrant (TSTQ) measured on days 44 to evaluate cognitive function. As with the memory test in the southwest quadrant, a platform was placed 2 cm underwater. A total number of four trials per day were taken. Before day 0, every animal was trained in different directions, starting from one release point. There was a particular gap of 15 min in each trial. The working memory was executed 30 s after hemorrhage induction on the day, and escape latency was also measured. When 10 s or 120 s had elapsed for the platformstationed rat, each reference and work memory test was completed. In 120 s, when the rats were not found, they were directed to stand on the platform, and then the latency of the escapewas recorded in 120 s. On day 44, rats were tested, and a TSTQ assessment was carried out, and the platform was removed [61].

On days 1st, 13th, 23rd, and 43rd days, the motor co-ordination ability of each animal were tested. The slip numbers were recorded in each study and the motor performance of rats was also measured on a scale of 0–4. An animal that could easily cross the beam was assigned a score of 0. Animals with mild, moderate, and severe disabilities were given scores 1, 2, and 3, respectively. The fourth score was given to the animals that could not go on the beam [62].

On day 1st, 13th, 23rd, and 43rd tests have been performed to assess the depressive performance of rats. The first exposure in the tank for rats takes 15 min during the training phase, while the second is done 24 h after the first exposure, with a 5 min exposure time. The test period was the 6-min exposure for rats, the first 2 min are a habit period and the last 4 min are a test itself which results in the duration of the immobility [63].